Tyrosine kinase containing Ig and EGF homology domains (TIE) ligands and nucleic acid molecules encoding them

摘要

Isolated nucleic acid molecules encoding the TIE ligands NL1, NL5, NL8, and NL4, the proteins encoded by such nucleic acid molecules, as well as methods and means for making and using such nucleic acid and protein molecules. Wherein; Seq ID 2 (NL1): MRPLCVTCWWLGLLAAMGAVAGQEDGFEGTEEGSPREFIYLNRYKR AGESQDKCTYTFIVPQQRVTGAICVNSKEPEVLLENRVHKQELELL NNELLKQKRQIETLQQLVEVDGGIVSEVKLLRKESRNMNSRVTQLYM QLLHEIIRKRDNALELSQLENRILNQTADMLQLASKYKDLEHKYQHL ATLAHNQSEIIAQLEEHCQRVPSARPVPQPPPAAPPRVYQPPTYNRIIN QISTNEIQSDQNLKVLPPPLPTMPTLTSLPSSTDKPSGPWRDCLQAL EDGHDTSSIYLVKPENTNRLMQVWCDQRHDPGGWTVIQRRLDGSV NFFRNWETYKQGFGNIDGEYWLGLENIYWLTNQGNYKLLVTMEDWS GRKVFAEYASFRLEPESEYYKLRLGRYHGNAGDSFTWHNGKQFTTLDR DHDVYTGNCAHYQKGGWWYNACAHSNLNGVWYRGGHYRSRYQDGV YWAEFRGGSYSLKKVVMMIRPNPNTFH Seq ID 4 (NL5): MKTFTWTLGVLFFLLVDTGHCRGGQFKIKKINQRRYPRATDGKEEAKKC AYTFLVPEQRITGPICVNTKGQDASTIKDMITRMDLENLKDVLSRQKREID VLQLVVDVDGNIVNEVKLLRKESRNMNSRVTQLYMQLLHEIIRKRDN SLELSQLENKILNVTTEMLKMATRYRELEVKYASLTDLVNNQSVMITL LEEQCLRIFSRQDTHVSPPLVQVVPQHIPNSQQYTPGLLGGNEI QRDPGYPRDLMPPPDLATSPTKSPFKIPPVTFINEGPFK DCQQAKEAGHSVSGIYMIKPENSNGPMQLWCENSLDPGGWTVIQKRTDGSV NFFRNWENYKKGFGNIDGEYWLGLENIYMLSNQDNYKLLIELEDW SDKKVYAEYSSFRLEPESEFYRLRLGTYQGNAGDSMMWHNG KQFTTLDRDKDMYAGNCAHFHKGGWWYNACAHSNLNGVWYRGGH YRSKHQDGIFWAEYRGGSYSLRAVQMMIKPID Seq ID 6 (NL8): MGKPWLRALQLLLLLGASWARAGAPRCTYTFVLPPQKFTGAVC WSGPASTRATPEAANASELAALRMRVGRHEELLRELQRLAAADGA VAGEVRALRKESRGLSARLGQLRAQLQHEAGPGAGPGA DLGAEPAAALALLGERVLNASAEAQRAAARFHQLDVKFRELAQLV TQQSSLIARLERLCPGGAGGQQQVLPPPPLVPVVPVRLVGST SDTSRMLDPAPEPQRDQTQRQQEPMASPMPAGHPAVPTKPVGPWQDCAEA RQAGHEQSGVYELRVGRHVVSVWCEQQLEGGGWTVIQRRQDGSVN FFTTWQHYKAGFGRPDGEYWLGLEPVYQLTSRGDHELLVLLEDWGGR GARAHYDGFSLEPESDHYRLRLGQYHGDAGDSLSWHNDKPFS TVDRDRDSYSGNCALYQRGGWWYHACAHSNLNGVWHHGGH YRSRYQDGVYWAEFRGGAYSLRKAAMLIRPLKL Seq ID 19 (NL4): MLKKPLSAVTWLCIFIVAFVSHPAWLQKLSKHKTPAQPQLKAANCC EEVKELKAQVANLSSLLSELNKKQERDWVSVVMQVMELESNSK RMESRLTDAESKYSEMNNQIDIMQLQAAQTVTQTSADAIYDCSSLY QKNYRISGVYKLPPDDFLGSPELEVFCDMETSGGGWTIIQRRKSGLV SFYRDWKQYKQGFGSIRGDFWLGNEHIHRLSRQPTRLRVEME DWEGNLRYAEYSHFVLGNELNSYRLFLGNYTGNVGNDALQYHNNTAFST KDKDNDNCLDKCAQLRKGGYWYNCCTDSNLNGVYYRLGEHNK HLDGITWYGWHGSTYSLKRVEMKIRPEDFKP