| 摘要 |
A solution containing double stranded nucleic acid is heated for dissociating the double stranded nucleic acid into single strands, which in turn are cooled and rebonded. A homoduplex and a heteroduplex are formed when the double stranded nucleic acid is a hetero body, while only a homoduplex is formed when the double stranded nucleic acid is a homo body. The temperature of the solution is increased for measuring ultraviolet absorption, thereby acquiring a thermal melting profile. When the heated and cooled solution contains only a homoduplex, the thermal melting profile has a single Tm temperature or two Tm temperatures close to each other. When the heated and cooled solution contains a homoduplex and a heteroduplex, the thermal melting profile has two separate Tm temperatures or further single Tm temperatures close to each other. Presence of a heteroduplex in sample double stranded nucleic acid as well as mutation in the base sequence thereof are detected on the basis of the Tm temperature(s) or the number thereof.
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