| 摘要 |
The present invention relates to methods and compositions to activate or modify the expression or activity of selected genes or gene pathways in cells. The invention more particularly relates to methods of preparing cells that express selected genes or gene pathways by mutagenesis in vitro, ex vivo, or in vivo. The invention more preferably discloses methods of modifying a regulator gene or sequence in a cell, so that the activity or expression of a selected gene or gene product is modified, without the structure of said gene product being altered. This invention can be used to produce improved cells for use in various areas such as screening, production, genetic analysis, cloning, cell therapy products, etc.
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