发明名称 |
RECOMBINANT PLASMID BEING CAPABLE OF EXPRESSING POLYHYDROXYALKANOATE(PHA) BIOSYNTHESIS ENZYME AND PHA DEPOLYMERASE AND METHOD FOR PRODUCING (R)-3-HYDROXYCARBOXYLIC ACID |
摘要 |
The present invention provides recombinant plasmids containing a gene coding for polyhydroxyalkanoate (PHA) biosynthesis enzyme and a gene coding for intracellular PHA depolymerase, and a process for preparing (R)-hydroxycarboxylic acid employing the same. In accordance with the present invention, optically pure (R)-hydroxycarboxylic acid can be prepared by culturing E. coli transformed with a recombinant plasmid which expresses intracellular PHA depolymerase of Ralstonia eutropha and PHA biosynthesis enzyme of Alcaligenes latus to give (R)-hydroxycarboxylic acid. The process for preparing (R)-hydroxycarboxylic acid of the invention can be successfully employed in large-scale continuous process with a high productivity by carrying out PHA synthesis and degradation in a simultaneous manner, while enjoying the benefits of simple procedures for harvesting cells and disposing the cell wastes. |
申请公布号 |
KR20010104925(A) |
申请公布日期 |
2001.11.28 |
申请号 |
KR20000026158 |
申请日期 |
2000.05.16 |
申请人 |
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
LEE, SANG YEOP;LEE, YEONG |
分类号 |
C12N15/09;C12N1/21;C12N9/18;C12N15/52;C12P7/42;C12P7/62 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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