INDICATOR OF FRESHNESS OF ROYAL JELLY

摘要

PROBLEM TO BE SOLVED: To provide an indicator of freshness of royal jelly using a protein in the royal jelly as an index. SOLUTION: This indicator of freshness of royal jelly is characterized in that the protein has the following properties. It is determined that the protein has a peak in about 25 min retention time by a high-performance liquid chromatography using TSK gel G 3000SW manufactured by Toso Co. Ltd. as a column and using 0.1M phosphate buffer solution (pH 7.0) containing 0.3M sodium chloride and 0.05% sodium azide as a developing solution and setting the flow rate of the solution to 0.3 ml/min and setting the column temperature to 35 deg.C and detecting at 280 nm absorbance. The indicator is represented by a value obtained by dividing a content of the indicator of freshness in the royal jelly with total protein in the royal jelly or a value obtained by multiplying the above value by 100.