High-throughput screens for enzyme inhibitors

摘要

The present invention provides a high-throughput screen for use in assaying for enzyme inhibitors of a target organism, comprising: a microorganism host in which an endogenous enzyme encoding gene has been replaced by a functionally complementing enzyme encoding gene from the target organism. The present invention also provides a method of identifying an inhibitor of a target organism enzyme, comprising the step of assaying a test inhibitor using a high-throughput screen comprising a microorganism host in which an endogenous enzyme encoding gene has been replaced by a functionally complementing enzyme encoding gene from the target organism.