| 摘要 |
An assay device for determining an analyte in an aqueous sample comprises: ( i) an elongate flow matrix (6) allowing lateral transport of fluid therethrough by capillary action, wherein the matrix comprises a liquid application zone (3) and downstream thereof, a detection zone (8) having an immobilized captu re agent capable of directly or indirectly binding to said analyte, (ii) a wicking member (13) placed at the downstream end of the flow matrix and havi ng an upstream end and a downstream end, and (iii) a time indicator (14) placed downstream of the detection zone (8) for indicating when liquid applied to t he liquid application zone has reached the time indicator. The time indicator comprises an indicator substance or substance combination which is capable o f exhibiting a visible colour change when hydrated by the aqueous sample. The assay device is characterized in that the time indicator (14) is arranged in contact with the wicking member (13) at a variable position between the upstream and downstream ends thereof to thereby permit variation of the time elapsing from the application of the liquid until the indicator substance changes colour. A method of performing an assay for determining an analyte i n a sample, comprises the steps of flowing sample and assay liquid(s) through the flowmatrix of the device such that they reach the detection zone in a predetermined sequence, and when the time indicator has changed colour, reading the result of the assay in the detection zone.
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