Visual image of ion distribution in tissue

摘要

A method is provided for producing a visual image of ions in a thin frozen tissue sample by placing the tissue sample in contact with a thin membrane having a water soluble gel or plastic thin film which has incorporated in or placed on its surface a fluorescent or color ion indicator which fluoresces or changes color under light of a particular wavelength. The thin frozen tissue sample is then subjected to light having a wavelength to cause the ion indicator to fluoresce or change color.