| 摘要 |
<p>A method of constructing a polynucleotide microarray whereby a sufficient amount of polynucleotide can be immobilized on a support and thus a uniform and strong signal can be obtained in a spot area even in the case of synthetic oligopolynucleotides which are known as being hardly immobilized via electrostatic bonds because of short chain length. Namely, a method of constructing a polynucleotide microarray characterized in that a solution in which a polynucleotide is dissolved in the step of immobilizing the polynucleotide on a support comprises water or a mixture of water with an organic solvent and is substantially free from any anionic electrolytes, and, in case where the solution comprises a mixture of water with an organic solvent, the content of the organic solvent in the solution is less than 50% by volume.</p> |
