发明名称 |
Solid phase synthesis |
摘要 |
A method of preparing an immobilized oligonucleotide having a free 3'-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3'-end and having a free 5'-end; ii) binding said oligonucleotide in a second position remote from the 3'-end to the solid support; and iii) selectively releasing the 3'-end of the oligonucleotide from the solid support to obtain the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3'-end.
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申请公布号 |
US6313284(B1) |
申请公布日期 |
2001.11.06 |
申请号 |
US19990423742 |
申请日期 |
1999.11.12 |
申请人 |
KWIATKOWSKI MAREK;LANDEGREN ULF;NILSSON MATS |
发明人 |
KWIATKOWSKI MAREK;LANDEGREN ULF;NILSSON MATS |
分类号 |
C12Q1/68;C07H21/00;(IPC1-7):C07H21/04;C07H21/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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