发明名称 |
NOVEL CELL SYSTEMS HAVING SPECIFIC INTERACTION OF PEPTIDE BINDING PAIRS |
摘要 |
This invention relates to novel modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide-binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; said fusion protein comprising a first peptide of a peptide binding pair, or segment of said first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a luciferase gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.
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申请公布号 |
WO0181548(A1) |
申请公布日期 |
2001.11.01 |
申请号 |
WO2001US13006 |
申请日期 |
2001.04.23 |
申请人 |
AMERICAN HOME PRODUCTS CORPORATION |
发明人 |
YOUNG, KATHLEEN, H.;CAO, JIAN |
分类号 |
C12N15/09;C07K14/39;C07K14/61;C07K14/72;C12N1/15;C12N1/19;C12Q1/02;(IPC1-7):C12N1/15;C12Q1/00;C12Q1/66;C12Q1/68;G01N33/53 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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