发明名称 |
MIPC COLUMN CLEANING SYSTEM AND PROCESS |
摘要 |
An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation soluti on supply means, and a separation solution conduit communicating with the separation column and the separation solution supply means, and a cleaning solution valve means positioned in the separation solution conduit for injecting cleaning solution into the separation solution conduit. A process for cleaning the non-polar DNA separation surfaces in the apparatus comprisi ng interrupting the flow of separation solvent with a block of cleaning solutio n injected into the flow of separation solution passing to the column, the cleaning solution containing agent which removes accumulated residues from t he non-polar surface. The cleaning solution can have an alkaline pH and contain a chelating agent such as EDTA.
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申请公布号 |
CA2406665(A1) |
申请公布日期 |
2001.11.01 |
申请号 |
CA20002406665 |
申请日期 |
2000.04.21 |
申请人 |
TRANSGENOMIC, INC. |
发明人 |
TAYLOR, PAUL D.;LAMB, KIMBERLY A.;GJERDE, DOUGLAS T.;HAEFELE, ROBERT M.;BUNNELL, LAURA |
分类号 |
G01N33/50;B01D15/08;B01D15/16;B01D15/20;B01D15/36;C12N15/00;G01N30/02;G01N30/26;G01N30/34;G01N30/50;G01N30/88;(IPC1-7):B01D15/08 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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