Culture medium for avian totipotential embryonic cells

摘要

Culture medium for totipotent, embryonic avian cells (embryonic stem cells, ESC) comprises a medium for avian cell culture supplemented with cytokines, fibroblast growth factor (FGF), insulin-like growth factor (IGF) and stem cell growth factor (SC). The medium is practically free of active retinoic acid. Also claimed are:(1) in vitro cultures of avian ESC produced using this medium;(2) ESC modified by introducing a gene that encodes a heterologous protein into the genome of an avian ESC in culture, and(3) transgenic animal derived at least partly from such cells.