摘要 |
PROBLEM TO BE SOLVED: To provide the principle of a solid phase PCR method by which a plurality of genes can simultaneously be amplified, to provide a method for performing the method, and to provide a technique for measuring the efficiency of the method. SOLUTION: This solid phase PCR method comprising a process for synthesizing plural pairs of oligonucleotide primers at the different sites of a solid phase or binding the plural pairs of oligonucleotide primers to the different sites of the solid phase, a process for performing the PCR reactions from the respective pairs of the immobilized primers on the solid phase, and a process for quantifying DNA from the PCR reaction products produced at the plural sites of the solid phase.
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