| 摘要 |
<p>L-Glutamate oxidase having the following physicochemical properties and gene thereof: (A) Function: L-glutamic acid + O2 + H2O → α-ketoglutaric acid + H2O2 + NH3. (B) Substrate specificity: being specific to L-glutamic acid. (C) Molecular weight and subunit structure: molecular weight determined by SDS-polyacrylamide gel electrophoresis: 70000 ± 6000, molecular weight determined by gel filtration: 140000 ± 10000. Being a dimer composed of the same subunits having a molecular weight of 70000 ± 6000. (D) Optimum pH: around pH 6.0 to 8.5. (E) Heat stability: being stable up to 60°C at pH 7.4 for 30 minutes. (F) Coenzyme: flavin adenine dinucleotide (FAD).</p> |
