NOVEL RECOMBINANT ANTIBODY, AMINO ACID SEQUENCES OF ITS COMPLEMENTARITY DETERMINING REGIONS AND GENES ENCODING THE SAME

摘要

An H chain polypeptide or its fragment of a recombinant antibody against hum an TNF.alpha. having at least one of the following amino acid sequences: a) as CDR-H1 Gly-Tyr-Thr-Phe-Thr-Asn-Tyr-Gly-Met-Asn; b) as CDR-H2 Trp-Ile-Asn-Thr - Tyr-Thr-Gly-Glu-Pro-Thr-Tyr-Ala-Asp- Asp-Phe-Lys-Gly; and c) as CDR-H3 Tyr-A sp- Tyr-Asp-Gly-Phe-Asp-Tyr; an L chain polypeptide of a recombinant antibody against human TNFa having at least one of the following amino acid sequences : a') as CDR-L1 Thr-Ala-Ser-Ser-Ser-Val-Ser-Phe-Ser-Tyr-Leu-His; b') as CDR-L2 Tyr-Ser-Thr-Ser-Asn-Leu-Ala-Ser; and c') as CDR-L3 His-Gln-Tyr-Leu-Arg-Ser-P ro- Tyr-Thr; and humanized antibodies against human TNF.alpha. or fragments thereof which comprise the above-described H chain polypeptide or its fragme nt and the above-described L chain polypeptide. Also, a process for producing a humanized anti-TNF.alpha. antibody by transforming host cells with an expression vector having a gene encoding the above antibody, etc. is disclos ed.