CODON OPTIMISATION FOR EXPRESSION IN RETROVIRUS PACKAGING CELLS

摘要

A method of producing a replication defective retrovirus comprising transfecting a producer cell with the following: iii) a retroviral genome; i v) a nucleotide sequence coding for retroviral gag and pol proteins; and iii) nucleotide sequences encoding other essential viral packaging components not encoded by the nucleotide sequence of (ii); characterised in that the nucleotide sequence coding for retroviral gag and pol proteins is codon optimised for expression in the producer cell.