METHOD FOR DETERMINING BASE SEQUENCE OF ANALYTICAL OLIGONUCLEOTIDES FOR THE DETECTION OF NUCLEIC ACIDS

摘要

Method for determining a nucleotide sequence of an analytical oligo nucleic acid for use in analysis of a nucleic acid, comprising the steps of listing all unit nucleotide sequences present on a target nucleic acid having a predetermined length which is shorter than that of the analytical oligo nucleic acid to be designed, and extracting a nucleotide sequence containing a sequence occurring at a low frequency on the target nucleic acid from the candidate sequences of the analytical oligo nucleic acid on the basis of a frequency of each of the listed individual unit sequences.