| 摘要 |
The present invention provides a process for producing a polypeptide delivery system comprising an ISCOM coupled to a polypeptide, the process comprising (a) mixing together the polypeptide, cholesterol, a saponin and a phospholipid in the presence of a nonionic detergent and a second detergent to form a solution of the polypeptide, cholesterol, phospholipid and saponin, and (b) removing detergent from the mixture so that the ISCOM forms, wherein the second detergent comprises the structure Z-X-Y, wherein X is uncharged and represents an optionally substituted hydrocarbon moiety having at least 8 aliphatic carbon atoms, Y represents a charged moiety and Z represents the remainder of the detergent structure. The second detergent can be selected from 3-(4-<i>tert</i>-octylphenoxy)-1-propanesulphonate, tetradecyltrimethylammonium, cetyltrimethylammonium and deoxycholate. The invention also relates to a polypeptide delivery system obtainable by the process, a vaccine comprising such a delivery system and the use of such a delivery system in the manufacture of a vaccine. |
