| 摘要 |
<p>The present invention provides an eukaryotic recombinant vector suited for bi-directional transcription of a transgene to yield both sense and antisense RNA transcripts of the transgene in an eukaryotic cell. The invention vectors are particularly suited for mediating gene silencing in a variety of biological systems. The present invention also provides host cells and transgenic plants comprising the invention vectors. Further provided by the invention are methods of inhibiting expression of an endogenous gene present in an eukaryotic cell. Also included is a method of identifying a biological function(s) of an endogenous gene of interest in an eukaryotic cell by selectively inhibiting the expression of the endogenous gene.</p> |
