| 摘要 |
<p>A process for producing a polypeptide delivery system comprising an ISCOM coupled to a Helicobacter pylori polypeptide or a fragment thereof, the process comprising (a) mixing together the polypeptide, cholesterol, a saponin and a phospholipid comprising a polar head group, in the presence of a detergent to form a solution of the polypeptide, cholesterol, phospholipid and saponin, and (b) removing detergent from the mixture so that the ISCOM forms, wherein in step (b) the head group of the phospholipid has a net charge and a terminal charge. The phospholipid can be phosphatidate or a derivative thereof. The invention also relates to a polypeptide delivery system obtainable by the process, a vaccine comprising such a delivery system and the use of such a delivery system in the manufacture of a vaccine.</p> |
