| 摘要 |
<p>The present invention relates to methods of detecting cells in a sample and to obtaining quantitative information about cell populations within a sample. In particular, the present invention provides a method of distinguishing between living and dead cells in a sample, said method comprising: (a) contacting the sample with a viability probe which modifies the nucleic acid of dead cells within the sample; and (b) detecting nucleic acid from the cells in the sample. Also described is a method of detecting cells in a sample, said method comprising: (a) contacting the sample with a viability probe which labels the nucleic acid of dead cells within the sample; (b) separating the nucleic acid from the cells into labelled and non-labelled fractions; and (c) detecting the nucleic acid in one or both of the fractions.</p> |
