| 摘要 |
<p>A method for detecting the presence of mutations in DNA samples by multiplexed electrophoresis, which method comprises: (a) introducing each of the DNA samples, all of which comprise a buffer solution comprising a matrix and at least one of which comprises heteroduplexed DNA, separately into a plurality of containers in an electrophoresis system; (b) heating the DNA samples independently of the electrical field in the electrophoresis system through at least one predetermined spatial or temporal temperature range selected to denature the DNA samples; and (c) detecting the presence of any mutations in the DNA samples by comparing an electrophoretic pattern for each of the DNA samples with an electrophoretic pattern for a control DNA sample, whereupon the presence of mutations in DNA samples is detected by multiplexed electrophoresis. The method can further comprise in (b) introducing at least one control DNA sample, which comprises a homoduplexed DNA in a buffer solution comprising a matrix, separately into a container in the plurality of containers in the electrophoresis system.</p> |
