IDENTIFICATION OF NOVEL PRO- AND ANTI-ANGIOGENIC AGENTS

摘要

Disclosed is a method for determining whether a compound is capable of binding to a receptor protein complex comprising Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) and Neuropilin-1 (NP-1), the method comprising introducing a sample comprising the compound to the VEGFR-2 + NP-1 protein complex. It is also an object of the present invention to provide a complex formed by the interaction of a recombinant VEGFR-2 receptor protein and a recombinant NP-1 receptor protein. Further disclosed are method for determining whether a test compound produces a signal upon binding to a VEGFR-2 + NP-1 protein complex (i.e. is an agonist for the complex), or blocks a signal upon VEGF165 binding to the complex (i.e. is an antagonist for the complex).