发明名称 |
METHOD OF SEPARATING HEMOGLOBIN A2 |
摘要 |
A method of separating hemoglobin A2 is provided which enables more precise and quicker separation of HbA2 than conventional methods. A method for separating hemoglobin A2 from a hemoglobin mixture according to a cation exchange liquid chromatography characterized that at least two types of eluents are used including an eluent (hereinafter referred to as "eluent A") for elution of faster hemoglobins than hemoglobin A0 and an eluent (hereinafter referred to as "eluent B") for elution of hemoglobin A0 and slower hemoglobins than hemoglobin A0, and that the eluent A has a pH of 4.0 - 6.0 and the eluent B is maintained at a pH of at least 0.5 higher than that of the eluent A. <IMAGE> |
申请公布号 |
EP1146333(A1) |
申请公布日期 |
2001.10.17 |
申请号 |
EP20000961173 |
申请日期 |
2000.09.22 |
申请人 |
SEKISUI CHEMICAL CO., LTD. |
发明人 |
SHIMADA, KAZUHIKO;SETOGUCHI, YUJI;OISHI, KAZUYUKI;KAWABE, TOSHIKI |
分类号 |
G01N30/26;B01D15/16;B01D15/36;B01J39/04;B01J39/26;G01N30/24;G01N30/34;G01N30/88 |
主分类号 |
G01N30/26 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|