PLANT PARASTIC SOIL NEMATOPHAGOUS MICROBIAL PREPARATION

摘要

PURPOSE: Provided is a microbial preparation using plant parastic soil nematophagous microorganism, Arthrobotrys sp. (KCTC 8992P) which is useful as a natural enemy of soil nematodes and can be applied to insecticides. CONSTITUTION: The microbial preparation is composed of 1-100 part by weight of Arthrobotrys sp. biomass based on 1000 part by weight of a pharmaceutical excipient which comprises defatted bran, silkworm powder and zeolite in a mixing ratio of 4-6:1:8-12. The plant parastic soil nematophagous microorganism, Arthrobotrys sp. (KCTC 8992P), is obtained by the steps of: sieving soil with a 1.0 mm sieve; suspending sieved soil with sterilized distilled water; centrifuging suspended solution and separating supernatant and precipitates; inoculating the precipitates on a corn meal 2 agar medium and culturing at 25 deg.C for 24 hours; adding 200-300 plant parastic nematophagous microorganisms thereto then culturing them at 25 deg.C for 7 days with aseptic culture technique to form spores of the plant parastic soil nematophagous microorganism; transplanting the spores to another corn meal 2 agar medium followed by culturing them; and re-culturing the culture with pure-culture technique, wherein a medium therefor is prepared by mixing 1.0-3.0 wt.% of peptone; 1.0-3.0 wt.% of beef-extract; 0.5-1.5 wt.% of yeast extract; 4.0-7.0 wt.% of glucose; 0.02-0.05 wt.% of KH2PO4; 0.003-0.008 wt.% of MgSO4; 0.003-0.008 wt.5 of KCl; and a balance of distilled water, and its pH is adjusted to pH 5-7 by using NH4OH.