| 摘要 |
A fluorescence-measuring device for scanning a specimen (36, 226) with a selection element (26, 206). Excitation light is coupled to an excitation beam path and guided to the specimen surface. Fluorescent light, which is emitted by the specimen surface, crosses the excitation beam path in a reverse direction and at the same time is decoupled from the excitation beam path. In the beam path, there is a first dispersive element (30, 210) so that the fluorescent light emitted by the specimen surface can strike the selection element spectrally split. The selection element includes a first area (48, 240) and a second area (26, 242) allowing selection between the excitation and fluorescent light. According to a first aspect, a spatial extension of a transmission area (26) is adjustable in order to adjust the transmitted wavelength range of the emission light. According to a second aspect, the selection element (206) makes it possible to operate with a coherent excitation having several laser lines. A two-dimensional confocal measurement arrangement can be accomplished and the emission light separated from the excitation light can be detected with spectral resolution.
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