发明名称 |
PROBE FOR CONSTRUCTING PROBE POLYMER, METHOD OF CONSTRUCTING PROBE POLYMER AND UTILIZATION THEREOF |
摘要 |
A method of assaying a target gene under isothermal conditions without using any enzyme. A double-stranded probe polymer is formed by hybridizing plural probe pairs, each consisting of a pair of probes having n (n >/= 3) base sequence domains complementary to each other, in such a manner as to form alternate crossing. The base sequence at the branching point of the complementary base sequence domains is bonded to a G (guanine)-C (cytosine) bond to thereby construct a stable double-stranded probe polymer. A complex of a target gene with the probe polymer is formed by using a base sequence complementary to a part of the target gene as one of the complementary domains of the probe and thus the target gene is assayed.
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申请公布号 |
WO0175157(A1) |
申请公布日期 |
2001.10.11 |
申请号 |
WO2001JP02554 |
申请日期 |
2001.03.28 |
申请人 |
SANKO JUNYAKU CO., LTD.;USUI, MITSUGU;HAKII, CHIKAKO;MITSUKA, MARI |
发明人 |
USUI, MITSUGU;HAKII, CHIKAKO;MITSUKA, MARI |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
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主权项 |
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