发明名称 |
METHOD OF AJMALINE PREPARING |
摘要 |
biotechnology. SUBSTANCE: invention relates to a novel method of preparing ajmaline in Rauwolfia serpentica tissue culturing. Rauwolfia tissue culturing is carried out in nutrient medium in the presence of bis-(hydroxymethyl)-phosphinic acid melamine salt (melaphene) taken in concentrations 1x10-3-1x10-5 g/l. The use of bis-(hydroxymethyl)-phosphinic acid melamine salt (melaphene) ensures to carry out the process both in dark and in light. Culturing Rauwolfia serpentina in nutrient medium in the presence of S-adenosyl-methionine taken in concentrations 7-15 mg/l results to increase of ajmaline yield. In the presence of both bis-(hydroxy- -methyl)-phosphinic acid melamine salt (melaphene) and S-adenosylmethionine the yield of the end product is increased by 2-fold. EFFECT: increased yield, constricted spectrum of biosynthesis products, decreased cost of production. 3 cl, 14 dwg, 4 tbl, 3 ex
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申请公布号 |
RU2174555(C1) |
申请公布日期 |
2001.10.10 |
申请号 |
RU20000118990 |
申请日期 |
2000.07.17 |
申请人 |
E. ARBUZOVA KAZANSKOGO NAUCHNOGO TSENTRA RAN;INST ORCH I FIZICHESKOJ KHIM I |
发明人 |
VINTER V.G.;FATTAKHOV S.G.;KOZLOVA R.JU.;REZNIK V.S.;KONOVALOV A.I. |
分类号 |
A61K36/24;A61K31/407;A61P9/06;C12N5/04;(IPC1-7):C12N5/04 |
主分类号 |
A61K36/24 |
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