| 摘要 |
Detecting and/or quantifying (M1) the binding of a first molecule (I) to a second molecule (II) comprising a solution containing (I) which is added to a vessel having (II) immobilized on its wall, incubating, and determining the change in concentration of (I) in the solution using radiation, without removal of the solution from the vessel, is new. Independent claims are also included for the following: (1) a method (M2) in which binding of (I) and (II) releases a fragment (III) from (II) and the change in concentration of (III) is measured; (2) detecting and/or quantifying (M3) the enzymatic or chemical activity of (I) towards (II) or other molecules with an affinity for (I); and (3) a microtiter plate for performing the new methods in which the wall, but not the base of the vessel is activated for binding of (II). |
