发明名称 |
Detection of disseminated tumor cells by quantitative RT-PCR of cytokeratin 20 mRNA |
摘要 |
<p>A method for the detection of cytokeratin 20 (CK-20) mRNA in the tissue or body fluid sample of a patient, which is characterized by i) isolating RNA from said sample; ii) preparing cDNA from said RNA by reverse transcription and dividing said cDNA into two compartiments; iii) performing polymerase chain reaction (PCR) with CK-20 specific primers in the first compartiment; iv) performing PCR with primers specific for a housekeeping gene in the second compartiment; v) calculating the relative ratio of mRNA of CK-20 to the housekeeping gene from the results of steps iii) and iv) as means for amount of the CK-20 mRNA in the sample, provides a sensitive and quantitative method for the detection of CK-20. <IMAGE></p> |
申请公布号 |
EP1138780(A1) |
申请公布日期 |
2001.10.04 |
申请号 |
EP20000107036 |
申请日期 |
2000.03.31 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
TABITI, KARIM, DR.;SAGNER, GREGOR, DR.;GUTEKUNST, MARTIN, DR.;SOONG, RICHIE, DR. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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