发明名称 |
PLASMID TO BE USED IN CHROMOSOME RECOMBINATION OF ESCHERICHIA COLI |
摘要 |
A technique of efficiently and artificially recombining a specific gene on t he chromosome of a microorganism belonging to the genus Escherichia which is highly useful as a bacterium producing various useful substances. A plasmid, which is replicable in Escherichia coli K-12 strain but non replicable in microorganisms belonging to the genus Escherichia other than the Escherichia coli K-12 strain, is constructed. A target gene is inserted into this plasmi d and then transferred to a microorganism belonging to the genus Escherichia other than the Escherichia coli K-12 strain. Thus, a strain having the substitution of the target gene can be efficiently selected.
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申请公布号 |
CA2405326(A1) |
申请公布日期 |
2001.10.03 |
申请号 |
CA20012405326 |
申请日期 |
2001.04.06 |
申请人 |
KYOWA HAKKO KOGYO CO., LTD. |
发明人 |
TAKANO, JUNICHI;FURUKAWA, SATORU;KINO, KUNIKI |
分类号 |
C12N1/21;C12N15/70;(IPC1-7):C12N15/70 |
主分类号 |
C12N1/21 |
代理机构 |
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