METHOD FOR DESULFURIZATION OF DIBENZOTHIOPHENE USING A RECOMBINANT PSEUDOMONAS PUTIDA STRAIN AS BIOCATALYST

摘要

The invention concerns the design and implementation of a method for desulfurization of dibenzothiophene, in which a recombinant strain of Pseudomonas putida KT2442 is used as biocatalyst, wherein the desulfurization genes dszA, dszC and dszB isolated from Rhodococcus erythropolis IGTS8 ATCC53968 coding for two monooxygenases and one desulfinase respectively and the gene hpaC of Escherichia coli W ATCC11105 coding for a flavin:NADH reductase have been cloned in said recombinant strain by constructing a plasmid with a broad host spectrum called pBG8. Said method considerably increases DBT desulfurization speed of the biocatalyst thereby improving its yield in the utilization thereof in biodesulfurization processes of oil and/or oil fractions.