The present method is useful for the identification of genes, ORF's and other nucleic acid molecules which are essential for the expression of a specific phenotype in microorganisms. The method employs <i>in vitro</i> transposition in conjunction with an chromosomal integration vector containing a specific gene or genetic element whose function is unknown. Subsequent transformation of a recombination proficient host with the vector and growth first under non-integrating conditions and then under integrating conditions, followed by a selection screen for either single or double crossover events results in transformants that may be subjected to phenotypic screens to determine gene function.
申请公布号
WO0171040(A2)
申请公布日期
2001.09.27
申请号
WO2001US09003
申请日期
2001.03.21
申请人
E.I. DUPONT DE NEMOURS AND COMPANY;SHARPE, PAMELA, L.;CHENG, QIONG;NAGARAJAN, VASANTHA