| 摘要 |
<p>A method of site-specifically and efficiently cleaving a single-stranded RNA. This cleaving method, which comprises hybridizing a target RNA with an antisense oligonucleotide 1 complementary to the 5'-end domain of the target part of the target RNA and another antisense oligonucleotide 2 complementary to the 3'-end domain of the target part of the target RNA to give a nucleic acid complex and then bringing this nucleic acid complex into contact with an ion, etc. capable of cleaving a single-stranded RNA, is characterized in that intercalaters are introduced into the 5'-end of the antisense oligonucleotide 1 and/or the 3'-end of the antisense oligonucleotide 2, the nucleic acid complex has a gap and a desired cleavage site exists in the gap. A kit usable in effecting this method and a gene therapy method are also provided.</p> |
