| 摘要 |
A tissue section (I) is spread out on the upper side of a membrane (II). The paraffin wax is removed and (I) is dried on (II), then incubated with a protease solution (III). An absorbent layer soaked with (III) is brought into contact with the underside of (II). Periodically, (III) is trickled over (I), or coated over it. (II) is washed and finally incubated with protein denaturing agent. Independent claims are also included for the following: (1) a membrane with proteins from a tissue section arranged on its upper surface. Topological arrangement of proteins on the membrane corresponds with the original topological arrangement in the tissue section, the membrane having been prepared as described; and (2) an apparatus comprising a casing (4), base (8) and optional removable cover (6). On the base (8) a layer (10) of absorbent material (12) is arranged, dimensioned for central arrangement with spacing from the wall (14). Its upper surface (18) carries the membrane (20). The protease solution (16) at the base, soaks the absorbent. Preferred features: The casing is trough-shaped. The layer is single or multilayer, comprising the same, or different absorbent materials.
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