| 摘要 |
The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, The invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application <i>in vivo</i> (e.g., on the cell surface or intracellularly) and <i>in vitro</i> (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).
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