A DETECTION METHOD OF PROTEIN-PROTEIN INTERACTION

摘要

A method of detecting an interaction between a protein X and a protein Y having a reporter function in eucaryotic cells, characterized by comprising modifying the protein X and/or the protein Y so that the proteins X and Y ar e expressed in cells in different localization patterns, co-expressing the proteins X and Y in eucaryotic cells, and then specifying the intracellar localization of the protein Y by using the reporter function as an indicatio n; and a method of detecting the inhibiting or promoting effect of a compound Z on the interaction of the proteins X and Y in eucaryotic cells.