| 摘要 |
<p>A process for the production of physiologically-active human interferon alpha from genetically engineered yeast, Pichia pastoris, comprising digesting, with an enzyme to produce a linearized plasmid, a plasmid having a promoter and operationally linked to a human interferon alpha gene in the absence of a fusion region; transforming Pichia pastoris cells with the linearized plasmid by homologous recombination to form Pichia pastoris clones; screening the Pichia pastoris clones for high interferon alpha expression to find a high interferon-yielding Pichia pastoris clone; growing the high interferon-yielding Pichia pastoris clone; and purifying physiologically-active human interferon alpha protein from the high interferon-yielding Pichia pastoris clones.</p> |
