PROCESS FOR PRODUCING POLYPEPTIDE HAVING DISULFIDE BOND

摘要

A DNA containing a DNA (Promoter) containing a promoter required in gene expression, an SD sequence (SD), a sequence (CWsp) encoding the signal peptide of a cell wall protein (CWP) originating in bacillus, a gene (Polypeptide) encoding a polypeptide having disulfide bond, and a gene (PDI) encoding protein disulfide isomerase, which are ligated to each other in, for example, the following manner Promoter-SD-CWPsp-Polypeptide-SD-CWPsp-PDI,to thereby enable the co-expression of the protein disulfide isomerase with the polypeptide containing disulfide bond; and a process for producing a polypeptide by using this DNA. Thus, the accurate disulfide bond formation ratio can be elevated in an expression system of a gene-recombinant polypeptide having disulfide bond.