发明名称 |
Fluorescence energy transfer substrates |
摘要 |
A method for the preparation of a fluorescence resonance energy transfer (FRET) substrate having donor and acceptor species on opposite sides of a proteolytic cleavage site and wherein the donor and/or acceptor species are attached via the side chain(s) of amino acid(s) therein. The method comprises contacting a reactive donor or acceptor species with a polypeptide substrate having the side chain(s) of amino acid(s) therein adapted for reaction with the reactive species and then contacting the substrate so obtained with a corresponding reactive donor or acceptor species. Novel FRET substrates so prepared and their use in assays to identify modulators of protease activity.
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申请公布号 |
US6291201(B1) |
申请公布日期 |
2001.09.18 |
申请号 |
US19950557005 |
申请日期 |
1995.11.17 |
申请人 |
ZENECA LIMITED |
发明人 |
GARMAN ANDREW JOHN |
分类号 |
C07K1/113;C07K14/00;C07K14/31;C12Q1/37;G01N33/533;(IPC1-7):C12Q1/37;C12N9/48;C12N9/50;G01N33/52 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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