| 摘要 |
The present invention relates to an herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of fruit Eugenia jambolina. The said product is prepared from the pulp of the fruit Eugenia jambolina comprising cleaning and drying the fruit to remove the extraneous material from the outer layer of the said fruit, deseeding the said fruit, soaking the de-seeded pulp in water, mixing the said mixture of water and pulp and keeping it overnight in controlled cooled conditions to maintain the activity of hypoglycemic compounds, filtering the said mixture, washing the residue with water to extract all the active compounds from the said mixture of water and pulp in the form of clear watery solution, reducing the water content by keeping the said clear watery solution in freezing conditions to get the residues in concentrated form, purifying the said concentrated product in the chromatography by using slurries of different columns. Buffers are added in the said purified mixture present in the said chromatography to elute the mixture of hypoglycemic compounds. The thin layer chromatography is then conducted of the said hypoglycemic compounds in the said mixture to get the said hypoglycemic compounds in the form of bands. The said bands are exposed in the iodine chamber for the clear demarcation of the said hypoglycemic compound bands. The said hypoglycemic compounds are then extracted to get the herbal therapeutic product to control the glucose level.
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