METHOD FOR ASSAY OF EXISTENCE RATIO OF HETEROGENEOUS INDIVIDUAL

摘要

PURPOSE: To provide a method for quantitative and accurate assay of the existence ratio of heterogeneous population. CONSTITUTION: This method for quantitative assay of the existence ratio of heterogeneous population is composed of the following steps: More than two samples are obtained from an organism population or its processed materials; their DNA is extracted; a reaction mixture containing DNA, a specific primer for a target gene and a specific primer for a control gene is prepared; the solution is subjected to quantitative PCR to measure the amount of each amplified product; the existence ratio of the different species in the organic population is determined on the basis of the amount of both amplified products; and confidence of the existence ratio is determined by the statistical treatment.