A METHOD FOR INCREASING THE IN VITRO PRODUCTION OF CYSTIC EMBRYOID BODIES FROM MAMMALIAN EMBRYONIC STEM CELLS

摘要

The invention, in the fields of medicine and molecular and cellular biology, relates to a method for promoting the success rate of in vitro production of cystic embryoid bodies from mammalian embryonic stem cells. The method of the present invention comprises the step of increasing the quantity of MAMA and/or of its homologues. An aspect of the invention consists in the use of MAMA and/or its homologues as agent of differentiation in an in vitro culture medium of mammalian embryonic stem cells. Another aspect of the invention is an embryonic stem cell line transfected with a sequence of nucleic acid encoding MAMA or with its homologous sequence of nucleic acid, and the cystic embryoid bodies therefrom.