| 摘要 |
<p>The invention, in the field of medecine and molecular biology, concerns a system to control the expression of a gene of interest, either in vitro or in vivo. It involves a first DNA sequence comprising a gene of interest linked in functional relation to a promoter, and a second DNA sequence comprising a second gene that encodes a polypeptide having a recombinant activity specific for target DNA sequences, and two of said target DNA sequences flanking one of the said two DNA sequences. According to the invention, the said second DNA sequence is located between the said promoter and said gene of interest. Another object of the invention is a DNA vector for the transfection of cells characterized in that it contains at least the system of the invention. The invention also concerns a self excision DNA cassette constituted by a DNA sequence flanked by target DNA sequences comprising at least a gene that encodes an inducible polypeptide having a recombinant activity specific for said target DNA sequences. This self-excision DNA cassette may be used as a blocking sequence that prevent the expression of a given gene under the control of a promoter.</p> |
