发明名称 |
MELANOMA DIFFERENTIATION ASSOCIATED GENE - 7 PROMOTER AND USES THEREOF |
摘要 |
The present invention provides for an isolated <i>Mda-7</i> promoter capable of directing transcription of a heterologous coding sequence positioned downstream therefrom, wherein the promoter is selected from the group consisting of: (a) a promoter comprising the nucleotide sequence shown in SEQ ID NO:1; (b) a promoter comprising a nucleotide sequence functionally equivalent to the nucleotide sequence shown in SEQ ID NO: 1; and (c) a promoter comprising a nucleotide sequence that hybridizes to a sequence complementary to the promoter of (a) or (b) in a Southern hybridization reaction performed under stringent conditions. The invention provides for a host cell comprising the recombinant expression construct as described herein. The invention provides for a method for expressing foreign DNA in a host cell comprising: introducing into the host cell a gene transfer vector comprising an <i>Mda-7</i> promoter nucleotide sequence operably linked to a foreign DNA encoding a desired polypeptide or RNA, wherein said foreign DNA is expressed. The invention further provides for a method for treating cancer in a subject suffering therefrom which comprises administering to the subject an effective amount of a pharmaceutical composition which comprises a recombinant expression construct comprising: (a) a nucleic acid molecule that encodes a selected polypeptide; and (b) an <i>Mda-7</i> promoter nucleotide sequence operably linked to the nucleic acid molecule of element (a), wherein the coding sequence will be transcribed and translated when in a host cell to produce the selected polypeptide, and the <i>Mda-7</i> promoter is heterologous to the coding sequence and a pharmaceutically acceptable carrier.
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申请公布号 |
WO0164921(A1) |
申请公布日期 |
2001.09.07 |
申请号 |
WO2001US06782 |
申请日期 |
2001.02.28 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
FISHER, PAUL, B.;MADIREDDI, MALAVI, T. |
分类号 |
A61K48/00;C12N15/85;(IPC1-7):C12N15/63 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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