发明名称 |
METHOD OF REVERSE TRANSCRIPTION USING BACILLUS STERAOTHERMOPHILUS OR THERMOACTINOMYCES VULGARIS DNA POLYMERASE |
摘要 |
The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. <i>Thermoactinomyces vulgaris</i> and <i>Bacillus stearothermophilus</i> possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided. |
申请公布号 |
WO0164954(A1) |
申请公布日期 |
2001.09.07 |
申请号 |
WO2001US06571 |
申请日期 |
2001.02.28 |
申请人 |
PROMEGA CORPORATION;GU, TRENT;HUANG, FEN;HARTNETT, JAMES, ROBERT |
发明人 |
GU, TRENT;HUANG, FEN;HARTNETT, JAMES, ROBERT |
分类号 |
C12N15/09;C12N9/12;C12N15/10;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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