| 摘要 |
<p>A blood-brain barrier reconstruction model which is highly useful in fundamental studies on the blood-brain barrier permeation mechanism, and screening drugs with central action based on the blood-brain barrier permeation mechanism, drugs suffering from problems concerning side effect on the center, drugs targeting various receptors expressed in cerebral capillary endothelial cells or drugs targeting disorders in cerebral capillary endothelial cells. This blood-brain barrier reconstruction model is prepared by cocultivating an immortalized cerebral capillary endothelial cell line originating in a transgenic rat having large T antigen gene of a temperature-sensitive mutant SV 40 tsA58 transferred thereinto with an immortalized astrocyte cell line originating in the same rat in a non-contact state, or cocultivating the above-described immortalized cerebral capillary endothelial cell line and the immortalized astrocyte cell line together with an immortalized capillary adventitial cell line originating in the same rat in a non-contact state.</p> |
