发明名称 |
MARKER FREE TRANSGENIC PLANTS: ENGINEERING THE CHLOROPLAST GENOME WITHOUT THE USE OF ANTIBIOTIC SELECTION |
摘要 |
The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.
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申请公布号 |
WO0164023(A1) |
申请公布日期 |
2001.09.07 |
申请号 |
WO2001US06275 |
申请日期 |
2001.02.28 |
申请人 |
AUBURN UNIVERSITY;UNIVERSITY OF CENTRAL FLORIDA;DANIELL, HENRY |
发明人 |
DANIELL, HENRY |
分类号 |
C12N15/82;(IPC1-7):A01H5/00;C12N5/04;C12N15/29;C12N15/31;C12N15/52 |
主分类号 |
C12N15/82 |
代理机构 |
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地址 |
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