METHOD OF REVERSE TRANSCRIPTION USING BACILLUS STERAOTHERMOPHILUS OR THERMOACTINOMYCES VULGARIS DNA POLYMERASE

摘要

The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are als o provided.